Catalogue
Human IgA lambda (dimer)
Catalog number: P 444| Product Type |
Proteins & Peptides |
| Units | 0.5 mg |
| Application |
ELISA |
Background
Purified human IgA1 lambda may be used as an IgA reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.
Product
Chromatographically purified Human IgA1 lambda, dimer isolated from human blood plasma. 0.5 ml solution of 1 mg/ml P 444. No preservative added.
This IgA1 consists mainly of dimers (as shown in non-reducing PAGE) and may contain traces of J chain.
Confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). Not less than 98% as determined by agar-gel electrophoresis.
Concentration: 1 mg/ml
Storage
After arrival store at +4°C. Prolonged storage may be at -20°C. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
Shipping Conditions: This product is shipped at ambient temperature.
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
Hansen IS, Hoepel W, Zaat SAJ, Baeten DLP, den Dunnen J. Serum IgA Immune Complexes Promote Proinflammatory Cytokine Production by Human Macrophages, Monocytes, and Kupffer Cells through FcαRI-TLR Cross-Talk. J Immunol. 2017;199(12):4124-4131. doi:10.4049/jimmunol.1700883